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Age-dependent shift in anti-GPI populations of H121/+L121/h mice. L chain allelic inclusion in relation to GPI-binding capacity in B220+ IgMa+ splenocytes (A) and immature B cells in bone marrow expressing the knock-in H chain (gated as IgMa+B220+AA4.1+) (D). (B) The proportion of populations “G,” “D,” and “E” among total B220+ splenocytes. Each diamond represents an individual mouse. (C) H chain usage in total B220+ splenocytes.
