Fig. 4.

Comparison of StAR and P450c17 protein levels by Western blots after rescue experiment. A rescue experiment was performed as described in Materials and Methods. StAR and P450c17 protein levels were assessed in two 4-month-old and two 15-month-old PACAP^−/− animals that received i.p. injections of 2 nmol of PACAP (+PACAP) and compared with the levels of the same proteins in two 4-month-old and 15-month-old testes from PACAP^−/− animals that received i.p. injections of water (+Water) as a control. Shown is the representative intensity of the blots seen for each group of animals.