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. 2006 Feb 24;103(10):3799–3804. doi: 10.1073/pnas.0600065103

Fig. 3.

Fig. 3.

Notch signaling in Wnt-1-HMECs and in primary HMECs. (A) RT-PCR analysis of mRNA expression of the endogenous Notch target genes HES-1 and HES-5 in the parental HMECs and Wnt-1-HMECs. All real-time RT-PCR values are expressed as relative arbitrary units after internal normalization for 18S rRNA. (B) Notch signaling activity in different Wnt-1-HMECs (P15–P18) and parental HMECs (P4 and P5) derived from different reduction mammoplasties (D, A, and E) assayed with reporter plasmids containing an artificial Notch/RBP-Jk-responsive promoter (pGAwt) or a control reporter (pGAmut) without RBP-Jk-binding sites, plotted after internal normalization. (C) Immunoblot of Notch signaling components, Dll1, Dll4, Jagged1, and Jagged2, as well as Notch3 and Notch4 in parental HMECs (P4 and P5) and Wnt-1-HMECs (P15–P18) derived from different reduction mammoplasties (D, A, and E).