Fig. 1.

Construction of the Rad transgene and Rad expression in the Rad-Tg mice. (a) Schematic structure of the Rad transgene. (b) Southern blot analysis of the two transgenic lines with the highest expression of Rad. Equal amounts of tail DNA digested with EcoRI were blotted with a 1.1-kb MCK probe. (c) Western blotting with anti-Rad serum of quadriceps muscle lysates from the same two lines. Line 51 was used for all studies. (d) Western blot analysis comparing Rad protein level in different muscles from two pairs of WT and Rad-Tg mice. (e) Comparison of Rad protein level in the heart, lung, and skeletal muscle of a WT and a Rad-Tg mouse by Western blotting.