Fig. 3.

Chromosomal replication in the WT and different DNA polymerases mutants. Flow-cytometric analyses of the DNA content of the MG1655 nal^R lacZΔP and its dnaE^ts, dnaE^ts polB, dnaE^ts polB umuDC, and dnaE^ts polB dinB derivatives after incubation at 42°C for 0 (▵), 1 (□), and 2 h (○) with an inhibitor of cell division (cephalexin). This figure represents the results of one of three experiments that all showed the identical pattern of the effects of different polymerases on chromosomal DNA content.