Fig. 4.

Antigen requirements for tolerance induction. C3H/HeJ mice received hepatic gene transfer with AAV vector-expressing hF.IX or LacZ transgenes (1 × 10¹¹ vg per mouse, first vector) followed by i.v. injection of Ad-hF.IX or Ad-LacZ vector (4 × 10¹⁰ particles per mouse, second vector) 6 weeks later. Levels of systemic hF.IX (A) and anti-hF.IX (B) were measured 1 month after the second gene transfer.