Fig. 5.

Adoptive transfer of CD4⁺ and CD4-depleted splenocytes from hepatic AAV-hF.IX-transduced (hatched bars in graphs) or naïve control (open bars) C3H/HeJ mice. (A) Experimental outline. Six weeks after hepatic AAV-hF.IX gene transfer, cells were transferred by tail vein injection into naïve mice of the same strain. Control groups were given splenocytes from naïve mice. After 36 h, all animals received tail vein injection of Ad-hF.IX vector (4 × 10¹⁰ particles per mouse; n = 4 per experimental group). Systemic hF.IX levels were measured 1 month (B) and 2 months (C) after adenoviral gene transfer. D depicts anti-hF.IX levels at 2 months. Results in B–D are averages ± SD.