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. 2006 Mar 14;103(12):4640–4645. doi: 10.1073/pnas.0509341103

Fig. 1.

Fig. 1.

Infection with wild-type MV, but not vMyxT5KO, dramatically induces phosphorylation level of Akt. HOS (human osteosarcoma) (A), human renal cancer 786-0 (B) and breast cancer MDA-MB435 (C) cells were mock-infected (M) or infected with either vMyxlac or vMyxT5KO at an MOI of 5. Human cancer cells were classified as type I (HOS), type II (786-0), or type III (MDA-MB435) based on the induction of phosphorylated Akt after MV infection. (D) In vitro Akt kinase assay. 786-0 cells were mock-infected (lane 1) or infected with either vMyxlac (lane 2) or vMyxT5KO (lane 3). Samples were collected at 4 h, and equal amounts of protein lysate (50 μg per reaction) were immunoprecipitated with anti-Akt antibody (2 μg per reaction), respectively. The immunoprecipitates were subjected to an in vitro kinase assay using histone H2B as the substrate.