Fig. 2.

The phosphorylation of Akt induced by MV infection is insensitive to PI3K/Akt inhibitors in a time-dependent manner. Human renal cancer cells (786-0) were pretreated with PI3K or Akt inhibitors (50 μM LY294002 or 10 μM AKT inhibitor IV) for 1 h, and then the cells were infected with vMyxlac (MOI of 5). Akt phosphorylated at both p-Akt Ser-473 and p-Akt Thr-308 sites were detected in cell lysates (50 μg per lane) by using immunoblotting at various time points (0–12 hpi). Total Akt levels were not changed and taken as a loading control.