Fig. 3.

M-T5 interacts with Akt under both transfection and infection conditions. (A) HEK293 cells were cotransfected with pcDNA3T7-MT5 and either pcDNA3HA-Akt1 or an empty vector for 24 h. Coimmunoprecipitations (Co-IP) were performed in kinase lysis buffer using anti-HA (12CA5) or anti-T7 to precipitate complexes. Immunoblot (IB) analysis in SDS/8% PAGE detected the resulting immune complexes with anti-T7 (Upper) or anti-HA (Lower), respectively. Expression of the transfected plasmids is shown in the left lane. (B) M-T5 interacts with endogenous Akt in 786-0 cells after MV infection. 786-0 cells were infected with vMyxlac (MOI of 5) and, at 12 h after infection, immune complexes were pulled down with anti-Akt and immunoblotted with anti-MT5 (Upper). Expression of M-T5 and endogenous human AKT is indicated (Lower).