Fig. 4.

Activation of Akt is required for virus infection and the formation of foci in MV. Human renal cancer cells (786-0) were transfected with an HA-tagged DN-Akt plasmid (black) or pcDNA3 vector alone (gray) for 12 h and then infected with MV (vMyxGFP) at MOIs of 0.01, 0.1, and 1 for 24 (not shown) or 48 (A) h, respectively. (A) Fluorescent foci were enumerated under fluorescent microscopy. (B) Inhibition of Akt signaling significantly reduces late viral gene expression. 786-0 cells were transfected with DN-Akt for 12 h and then infected with vMyxGFP at an MOI of 1 for 24 h. The expression of DN-Akt results in a subsequent decrease in the expression of the late viral gene Serp1 and levels of p-Akt Ser-473, but the early gene T7 expression is not affected. Actin was used as a loading control.