Fig. 2.

Genes located in the SO1427–SO1432 gene cluster are anaerobically induced and specifically required for anaerobic growth by using DMSO. (A) Quantitative RT-PCR expression analysis of representative genes within gene clusters containing homologues of dmsAB in S. oneidensis. Expression of each gene was normalized to recA. (B) Anaerobic growth of dmsB (SO1430, ♦) and dmsE (SO1427, ▴) deletion strains were compared with a mutant containing a polar insertion in SO1427 (▾) and to wild-type MR-1 (■) using DMSO as the sole anaerobic electron acceptor. Error bars represent data range of duplicate cultures in all experiments.