Fig. 1.
RSK2 interacts with 5-HT2A receptors. (A) Protein sequence of mouse RSK2 (GenBank accession no. NP_683747), with the N-terminal kinase (NTK) domain of RSK2 (blue), the linker domain (red), the C-terminal kinase (CTK) domain (purple), and the i3 loop-binding region identified in the yeast two-hybrid screen (yellow). (B) Domain structure of RSK2, residues phosphorylated in RSK2 activation, and kinases known to phosphorylate those residues. (C) Growth of yeast strain PJ69–2A cotransformed with truncation mutants of the i3 bait and the RSK2 target on quadruple or triple dropout media (see Supporting Text for details).