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. 2006 Mar 10;103(12):4717–4722. doi: 10.1073/pnas.0600585103

Fig. 4.

Fig. 4.

RSK2 exerts a tonic brake on GPCR signaling. (A) The expression of RSK2 in the RSK2−/− fibroblasts, in the RSK2+/+ fibroblasts, and in the RSK2−/− RSK2-transfected stable line. (B) PI hydrolysis responses to various concentrations of 5-HT in RSK2−/− and RSK2+/+ fibroblasts and in RSK2−/− fibroblasts stably transfected with wild-type RSK2. (C) PI hydrolysis responses to various concentrations of TRAP in RSK2−/− and RSK2+/+ fibroblasts and in RSK2−/− fibroblasts stably transfected with wild-type RSK2. (D) PI hydrolysis responses to various concentrations of bradykinin in RSK2−/− and RSK2+/+ fibroblasts and in RSK2−/− fibroblasts stably transfected with wild-type RSK2. (E) PI hydrolysis responses to various concentrations of ATP in RSK2−/− and RSK2+/+ fibroblasts and in RSK2−/− fibroblasts stably transfected with wild-type RSK2. PI hydrolysis data are normalized to dpm per mg of total protein with RSK2+/+ fibroblast maximal signaling adjusted to 100%. (F Upper) A representative immunoblot of immunoprecipitated FLAG-5-HT2A receptors. (F Lower) A representative autoradiogram of cells metabolically labeled with [32P]orthophosphate followed by FLAG immunoprecipitation (IP) of FLAG-5-HT2A receptors. IB, immunoblot.