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MLE-12 cells express TRPV4. (A) Total RNA was harvested from MLE-12 cells, and RT-PCR was performed by using primers specific to 18S ribosomal RNA (18S control), TRPV1, TRPV2, or TRPV4, and products were visualized on an agarose gel. (B) Cell lysates from HEK control, TRPV-expressing cells, or MLE-12 cells were probed for TRPV4.
