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TRPV4 is required for the hypotonic reduction in AQP5 abundance. HEK control or TRPV4-expressing cells were transiently transfected with a control plasmid or AQP5. Thirty-six hours after transfection, cells were incubated in medium of the designated osmolality for 30 min in the presence or absence of RR, and immunoblots of cell lysates were probed for AQP5.
