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Detection of the Δnd4 mutation by PCR amplification. PCR on 12 transformants (D1 to D12) was performed by using primers nd4-F and nd4-R. D2 and D6 present the 69-bp NdeI deletion in nd4 at the heteroplasmic (D2) and homoplasmic (D6) state. The 100-bp ladder (Eurogentec, Liège, Belgium) is shown at the right.
