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. 2006 Mar 10;103(12):4777–4782. doi: 10.1073/pnas.0511066103

Fig. 6.

Fig. 6.

pLHCII interacts with CAO during import into wild-type but not ch1-3, chloroplasts. (A) Detection of plastid-envelope proteins interacting with pLhcb1/Lhcb1-(His)6 [pLHCII/LHCII-(His)6] in wild-type chloroplasts at different Mg-GTP and Mg-ATP concentrations. (B) Time course of pLhcb1-(His)6 import into wild-type (open circles) and ch1-3 (filled circles) Arabidopsis chloroplasts. Percentages refer to input radioactivities, set as 100%. (C) Purification of plastid-envelope proteins interacting with pLhcb1-(His)6 at different stages of import into wild-type and ch1-3 mutant chloroplasts. Lanes 1 and 2 show products formed after 15 min, whereas lanes 3–5 show products obtained at the indicated time intervals. (D) Toc159, Toc75, Toc34, CAO, and Ptc52 levels in envelopes of ch1-3 mutant and wild-type chloroplasts. Note the lack of Ptc52 in the recovered envelope complexes but its detection in OM–IM junction complexes containing pPORA used as control (Ctr).