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. Author manuscript; available in PMC: 2006 Apr 28.
Published in final edited form as: Anal Biochem. 2005 Sep 15;344(2):183–192. doi: 10.1016/j.ab.2005.06.032

Table 1.

Optical properties of screened aldehydes and common probes

Aldehyde and common probe UV absorption
Fluorescence
λmax (nm) εmaxa Ex (nm)b Emmax (nm)c Stokes' shift (nm) Relative intensityd
2-Naphthaldehyde 292 12.7 344 444 100 100
6-Methoxy-2-naphthaldehyde 314 19.1 330 460 130 260
Pyrene-1-carbaldehyde 397  9.8 397 470  73 246
9H-Fluorene-2-carbaldehyde 318 34.6 330 409  79 188
6-Dimethylamino-2-naphthaldehyde 377 18.4 396 528 132 173
Anthracene-9-carbaldehyde 408  6.7 410 519 109 106
10-Methyl-anthracene-9-carbaldehyde 425  9.1 399 531 132  26
Phenanthrene-9-carbaldehyde 318 13.2 334 471 137  25
10-Chloro-antharacene-9-carbaldehyde 437  6.0 410 532 122  22
3-(4-Nitro-phenyl)-propenal 308 27.0 397 460  63  9
3-(4-Dimethylamino-phenyl)-propenal 399 41.1 397 494  97  5
4-Quinoline-carbaldehyde 315  3.6 335 430  95  5
5-(4-Dimethylamino-phenyl)-penta-2,4-dienal 409 31.1 418 593 175  5
2-Quinoline-carbaldehyde 301  5.7 346 420  74  4
4-Dimethylamino-benzaldehyde 354 33.7 330 410  80  3
3-Quinoline-carbaldehyde 292 10.6 345 430  85  2
Resorufin 571 64.55 571 581  10 275
7-Hydroxy-4-trifluoromethyl coumarin 341 11.11 356 496 140 276

Note. The data were obtained in 0.1 M sodium phosphate buffer (pH 7.8).

a

Molar absorptivity (103 M−1 cm−1).

b

Ex: optical excitation wavelength.

c

Emmax: maximal emission wavelength.

d

Relative intensity: fluorescent intensity of 2-NA is 100.