Table 4.
Apparent O-dealkylation activity of substrates by microsomes of different species
| Treatment | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 7-Ethoxyresorufin | |
|---|---|---|---|---|---|---|---|---|---|---|
| Rat (Fisher) | None | 710 ± 30a | 410 ± 25a | 533 ± 3a | 544 ± 16a | 473 ± 35 | 378 ± 20 | 140 ± 4 | 160 ± 8 | 97 ± 8 |
| Rat (Wistar) | None | 1400 ± 300 | 570 ± 90 | 610 ± 50 | 420 ± 70 | 900 ± 100 | 1100 ± 200 | ND | ND | 70 ± 19 |
| Human | None | 147 ± 19 | 305 ± 21 | 353 ± 19 | 332 ± 15 | ND | ND | ND | ND | 50 ± 5 |
| CYP 1A1b | None | ND | ND | ND | ND | 106 ± 10 | 60 ± 2 | 30 ± 1 | 30 ± 2 | 23 ± 6 |
| Murine | Controlc | 139 ± 4 | 330 ± 27 | 232 ± 32 | 270 ± 27 | ND | ND | ND | 122 ± 2 | 52 ± 4 |
| BNFd | 143 ± 13 | 745 ± 46 | 223 ± 33 | 592 ± 96 | ND | ND | ND | 203 ± 11 | 324 ± 14 | |
| PBe | 113 ± 8 | 636 ± 16 | 126 ± 18 | 172 ± 23 | ND | 134 ± 3 | ND | 244 ± 25 | 66 ± 5 |
Note. Data are the means and standard deviations of three replicates (in pmol/min/mg). ND, not detected (data were below the limit of detection that was considered as corresponding to a signal-to-background ratio of approximately 3).
a
Rat (Fisher) data from Zhang and coworkers [14].
b
CYP 1A1 was expressed in insect cells using baculovirus [16].
c
Control rats were treated by saline or corn oil.
d
BNF: β-Naphthoflavone (80 mg/kg body weight).
e
PB: Phenobarbital (50 mg/kg body weight).