. Author manuscript; available in PMC: 2006 Apr 28.

Published in final edited form as: Anal Biochem. 2005 Sep 15;344(2):183–192. doi: 10.1016/j.ab.2005.06.032

Table 5.

Apparent kinetic constants for α-cyano ether substrates

Treatment	1		2		3		4		5		6
	V_max^a	K_m^b	V_max	K_m	V_max	K_m	V_max	K_m	V_max	K_m	V_max	K_m
Control^c	1.6 ± 0.1	0.59 ± 0.12	0.6 ± 0.1	0.85 ± 0.21	0.7 ± 0.1	0.60 ± 0.27	0.5 ± 0.1	1.62 ± 0.56	2.7 ± 0.1	19.6 ± 0.3	1.8 ± 0.1	13.4 ± 0.3
MC^d	31.5 ± 0.9	0.29 ± 0.04	21.6 ± 1.4	0.12 ± 0.03	11.9 ± 0.4	0.13 ± 0.01	5.4 ± 0.2	0.19 ± 0.02	4.5 ± 0.1	20.2 ± 0.2	3.4 ± 0.1	19.7 ± 0.5
PB^e	22.6 ± 0.8	0.20 ± 0.02	44.2 ± 3.7	0.13 ± 0.04	24.0 ± 1.7	0.42 ± 0.10	13.2 ± 1.1	0.25 ± 0.07	3.0 ± 0.1	18.7 ± 0.3	2.5 ± 0.1	19.6 ± 0.9

Note. Data are from Wistar rat liver microsomes. Activities of substrates 7 and 8 could not be detected. The kinetic data of 7-ER were not determined due to too low activity. Data are the means and standard deviations of three replicates.

V_{max app} values are expressed as nanomoles/minute/milligram protein.

K_{m app} values are expressed in micromolars.

Control rats were treated by saline or corn oil.

MC: 3-methylcholanthrene (75 mg/kg body weight).

PB: phenobarbital (80 mg/kg body weight).