Table 1.
The primer and probe sequences used in TaqMan PCR
| Assay | Primer | Sequence |
| ACTB | PubMed accession number: BC002409 | |
| Forward | 5' TGCCGACAGGATGCAGAAG 3' | |
| Reverse | 5' GCCGATCCACACGGAGTACT 3' | |
| Probe | FAM 5' AGATCAAGATCATTGCTCCTCCTGAGCGC 3' TAMRA | |
| TNFA | PubMed accession number: X01394 | |
| Forward | 5' CACGCTCTTCTGCCTGCTG 3' | |
| Reverse | 5' GATGATCTGACTGCCTGGGC 3' | |
| Probe | FAM 5' CCAGAGGGAAGAGTTCCCCAGGGAC 3' TAMRA | |
| IL18 | PubMed accession number: BC007461 | |
| Forward | 5' ATCGCTTCCTCTCGCAACA 3' | |
| Reverse | 5' CATTGCCACAAAGTTGATGCA 3' | |
| Probe | FAM 5' CAGGAATAAAGATGGCTGCTGAACCAG 3' TAMRA | |
Primers and probes were generated by Primer Express 1.0 using sequence data from Pubmed. All assays cross exon splicing points in order to avoid detection of genomic DNA. Following assay design, specificity was double-checked by a BLAST search on the NCBI website.