Figure 3.
Validation of the temporal Hotelling T2 -test with quantitative PCR experiments (1).The expression patterns of Dlk1 and Casq2 (significant in both temporal and non-temporal tests), and Dpp4 (significant in temporal but not in non-temporal test), as determined by microarray and qPCR assays, are plotted. The left panel shows the log-ratios with respect to time point 1 estimated from the microarray data; the middle panel shows the fitted second order polynomials; the right panel shows the log-ratios determined by quantitative PCR, as an independent confirmation method for the microarray data. X-axis represents time points. Solid lines represent WT mice; dashed lines MDX mice; dotted lines BSG mice; dashed line interrupted with dots GSG mice.