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TSLP does not induce Foxp3 expression in CD4+25- spleen T cells. CD4+25- T cells purified from the spleen were cultured with or without TSLP (100 ng/ml) for 2 days and analyzed for relative expression of FoxP3 (FoxP3/18S) by real-time PCR. Error bars indicate SD derived from duplicate samples. Shown are data repeated in at least three independent experiments.
