Table 1.
# Cells (103) | day 1 | day2 | day 3 | day 4 | day 5 | day 6 | day 8 | Adult |
Total Thymocyte | 1,005 b +/-177 | 3,390 +/-410 | 6,210 +/-127 | 9,410 +/-2,107 | 32,600 +/-18,668 | 31,000 +/-12,021 | 57,700 +/-2,263 | 75,900 +/-12,304 |
CD4+CD8- | 13 +/-1.9 | 117 +/-36.4 | 457 +/-44.5 | 350 +/-16.2 | 828 +/-526 | 732 +/-361 | 1,738 +/-256 | 5,606 +/-1,751 |
CD4+CD25+CD8- | 0.1 (.82%)C +/-0.02 | 0.9 (.86%) +/-0.6 | 3.1 (.86%) +/-0.3 | 2.6 (.92%) +/-0.2 | 3.7 (.76%) +/-2.2 | 4.5 (.98%) +/-1.9 | 18.1 (.86%) +/-0.3 | 91.1 (2.2%) +/-27 |
Foxp3+CD4+CD25+CD8- | 0 | 0 | 0.8 (21%)d +/-0.1 | 0.9 (37%) +/-0.1 | 1.6 (43%) +/-0.9 | 1.9 (43%) +/-0.7 | 10.8 (59%) +/-0.1 | 64.5 (71%) +/-17.9 |
a Total thymocytes from day 1–8 newborn or adult mice were stained with CD4, CD8, CD25 and anti-FoxP3 antibody and analyzed by FACS. Data is a summary of three independent analyses. b: Average cell number +/- standard deviations (SD). c: % CD25+ of CD4+CD8- thymocytes. d: % FoxP3+ of CD4+CD25+CD8- thymocytes.