TABLE 2.

PCR and sequencing primers forAMT4

Primer	Sequence (5′–3′)	Position in DNAa	Position in RNAa
−1577F	ATGCAGCCATGGTGGGAGTGC	−1577–−1557
ATGF	ATGGCGGACGAGATGGATCC	1–20	1–20
ATGR	TCATGGGATCCATCTCGTCC	25–6	25–6
500F	TTGCTCAGCTGCAGATCAAACG	477–498
500R	TCGTTTGATCTGCAGCTGAGC	499–479
TILL1F	AACAACCCCAACGGCTTTGTG	700–720
TILL1R	AAAGCCGTTGGGGTTGTTGC	717–698	360–341
TILL2F	CTCAACTTCAACGCCTACCTC	1089–1109	505–525
TILL2R	TGAGGTAGGCGTTGAAGTTGAGG	1110–1088	526–504
928F	TGCCTGTCCTGCCTGCTCACC	1790–1810	928–948
1195R	AGCAGGCCGGCAAAGAACACG	2058–2038	1196–1176
Short1F	ATCGGCATCATCTCCATCTTCG	2392–2413	1303–1324
Short1R	AGATGGAGATGATGCCGATCACC	2410–2388
Short2F	ATTGGACTTTCTGCTCATGG	3484–3503	2066–2085
UGA-1R	GTGCTTTCCATCTGAGCACC	3732–3713	2314–2295
EndR	TTTGGACGGCAGACTGTGCG	4009–3990	2591–2572

^aRelative to ATG translational start. Positions of the primers are also shown in Figure 6A.