TABLE 2.

Plasmids used in this study

Plasmid	Description	Source
pAR100-RVS-SH3 (BA1267)	RVS167 SH3 (codons 423–482) with an N-terminal 6-His tag under   the control of the T7 promoter	This work
pAR100-RVS-P473L   (BA1268)	Same as pAR100-RVS-SH3 but encoding a protein with leucine   substituted for proline at position 473	This work
pDN108 (BA1269)	fyn SH3 (codons 84–142) with a C-terminal Flag epitope followed by   a 6-His tag under the control of the T7 promoter	Maxwell and Davidson (1998)
pGPA-SH3-His (BA1344)	RVS167 GPA-SH3 (codons 282–482) with a C-terminal 6-His tag under   the control of the T7 promoter	Friesen et al. (2003)
pRSET-B-GYL1 (BA1612)	GYL1 with a 6-His tag under the control of the T7 promoter	This work
pET28 + GYP5 (BA1677)	Full-length GYP5 (residues 1–894) with an N-terminal 6-His tag under   the control of the T7 promoter	This work
pET28 + GYP5ΔN (BA1679)	GYP5 residues 403–894 with an N-terminal 6-His tag under the control   of the T7 promoter	This work
pET28 + GYP5ΔC (BA1680)	GYP5 residues 1–690 with an N-terminal 6-His tag under the control   of the T7 promoter	This work
pET19 + GYP5 (BA1343)	GYP5 missing the first 15 amino acids with an N-terminal 6-His tag   in pET19b	This work
pGEX + GYL1 (BA1334)	GYL1 with an N-terminal GST tag in pGEX-3X	This work
pET15-SEC4	SEC4 with an N-terminal 6-His tag in pET15b	Du et al. (1998)
pET15-YPT1	YPT1 with an N-terminal 6-His tag in pET15b	Du et al. (1998)
pET15-GYP1	GYP1 with an N-terminal 6-His tag in pET15b	Du et al. (1998)
p426-GAL	High-copy URA3 plasmid with GAL promoter	Ronicke et al. (1997)
pAcGHLT-RVS167	RVS167 with N-terminal GST + His tag used for insect cell infection	Friesen et al. (2003)
pAcHLT-RVS161	RVS161 with N-terminal His tag used for insect cell infection	This work
p426-GAL-GYL1-Flag   (BA1691)	GYL1 with a C-terminal Flag tag under control of the GAL promoter   on high-copy URA3 plasmid	This work
p415-GAL-GYP5-Flag   (BA1699)	GYP5 with a C-terminal Flag tag under control of the GAL promoter   on CEN LEU2 plasmid	This work