Figure 1.—

Histone H2A S122 is important for survival in the presence of MMS. (A) Schematic showing the amino acid sequence of the S. cerevisiae histone H2A C-terminal tail. Residues that were changed to alanine are indicated by arrows. Serine 129 is underlined. (B) Serial dilutions of hta1-mutant yeast strains and their isogenic wild-type control (FHY2) grown with or without 0.03% MMS. (C) Growth of wild-type (diamonds) and hta1-S122A mutant (triangles) cultures grown at 30° was monitored by changes in OD_600nm. (D) Survival of wild-type and hta1-S122A mutant yeast on MMS-containing plates. (E) Western blot analysis with antihistone H2A antiserum (top left) and Coomassie-stained SDS polyacrylamide gel (bottom left) of yeast lysates prepared from wild-type (lane 2) and hta1-S122A mutant strains (lane 3). Lane 1 contains molecular weight markers. Western blot analysis with anti-H2A and anti-Tip49a antibodies (right).