Figure 3.—

Histone H2A S122 is not required for DNA damage checkpoint responses or gross chromatin structure. (A) Western blot analysis of wild-type (FHY2) and hta1-S122A lysates prepared from cultures grown with or without 0.1% MMS and analyzed using anti-Rad53 antiserum. (B) Wild-type (diamonds) and hta1-S122A mutant (triangles) cultures were arrested in G₂/M using nocodazole and incubated with (open symbols) or without (solid symbols) 0.1% MMS. Cultures were released and the percentage of large-budded cells was calculated at the indicated time points. (C) An ethidium-bromide-stained gel of chromatin isolated from wild-type or hta1-S122A mutant yeast that was digested with MNase for various periods of time (0, 1, 2, 5, 10, or 20 min).