Figure 5.—

Histone H2A S122 is important for sporulation and survival in the presence of DNA double-strand breaks. (A) Sporulation levels of wild-type and hta1-S122A mutant cells after 5 days in sporulation media. (B) Serial dilutions of haploid (H) or diploid (D) wild-type and hta1-S122A mutant yeast grown in the presence or absence of MMS. (C) Survival of JDY94 complemented with wild-type HTA1 (wild type) or an empty vector (hta1-S122A) in the presence of continual HO endonuclease expression. Survival is presented as a percentage of colonies grown on galactose-containing media compared with strains grown on glucose-containing media. (D) Survival of JDY94 complemented with wild-type HTA1 (wild type) or an empty vector (hta1-S122A) in the presence of EcoRI expression. Survival is presented as a percentage compared with strains lacking the EcoRI endonuclease construct.