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. 2005 Jun;170(2):543–553. doi: 10.1534/genetics.104.038570

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Copyright © 2005, Genetics Society of America

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Figure 6.— — Histones H2A S122 and S129 provide distinct functions. (A) Western blot of wild-type (FHY2) and hta1-S122A mutant lysates prepared from cultures grown with or without 0.1% MMS. (Top) Lysates analyzed with antibody against histone H2A phospho-S129. (Bottom) Lysates analyzed using antihistone H2A antiserum. (B) Survival of indicated hta1-mutant strains and their isogenic wild-type control on medium containing indicated concentrations of MMS. (C and D) Serial dilutions of midlog cultures of the indicated strains plated onto medium containing the indicated agents. (E) Serial dilutions of midlog cultures of haploid strains harboring two H2A-encoding plasmids were plated onto selective medium containing the indicated concentration of MMS. (F) Serial dilutions of midlog cultures of the indicated strains grown in the presence or absence of MMS.