TABLE 1.
Yeast strains used in this study
| Strain | Genotype | Plasmid (relevant sequences) | Reference |
|---|---|---|---|
| FY406 |
MATa (hta1-htb1)Δ::LEU2 (hta2-htb2)Δ:: TRP1 ura3-52,1 leu2Δ1 lys2Δ1 lys2-128Δ his3Δ200 trp1Δ63 |
pAB6 (HTA1-HTB1, URA3) | Hirschhorn et al. (1995) |
| FHY2 | As FY406 | pJD150 (HTA1-HTB1, HIS3) | Downs et al. (2000) |
| FHY3 | As FY406 | pJD151 (hta1-S129A-HTB1, HIS3) | |
| FHY44 | As FY406 | pJD187 (hta1-T103A-HTB1, HIS3) | This study |
| FHY45 | As FY406 | pJD188 (hta1-K120A-HTB1, HIS3) | This study |
| FHY46 | As FY406 | pJD189 (hta1-K121A-HTB1, HIS3) | This study |
| FHY47 | As FY406 | pJD190 (hta1-S122A-HTB1, HIS3) | This study |
| FHY48 | As FY406 | pJD191 (hta1-K124A-HTB1, HIS3) | This study |
| FHY49 | As FY406 | pJD192 (hta1-T126A-HTB1, HIS3) | This study |
| FHY50 | As FY406 | pJD193 (hta1-K127A-HTB1, HIS3) | This study |
| FHY54 | As FY406 | pJD197 (hta1-S122A/S129A-HTB1, HIS3) | This study |
| FHY58 | As FY406 | pJD210 (hta1-S122E-HTB1, HIS3) | This study |
| FHY12 | Diploid of FHY2 | pJD150 (HTA1-HTB1, HIS3) | This study |
| FHY69 | Diploid of FHY47 | pJD190 (hta1-S122A-HTB1, HIS3) | This study |
| JDY1 |
MATaura3-52 trp1Δ leu2-3, 112 his3Δ200 Δrad52::TRP1 |
Downs et al. (2000) | |
| Y865 | MATaura3-52 trp1-289 his3Δ1 leu2-3,112 gal2 gal10 | Costigan et al. (1994) | |
| Y869 | MATaura3-52 trp1-289 his3Δ1 leu2-3,112 gal2 gal10 nhp6A-Δ2::URA3 nhp6B-Δ3::HIS3 | Costigan et al. (1994) | |
| JDY94 | hta1-S122A; (hta2-htb2)Δ::TRP1 | This study |