Figure 2.—

Genomic distribution of repetitive DNA sequences obtained after FISH on flow-sorted chromosomes. Labeled DNA probes were detected with either fluorescein (yellow-green) or Cy3 (red). Chromosomes were counterstained with either propidium iodide (red) or DAPI (blue). For each chromosome, two representative examples are given. (A) Distribution of three repetitive DNA sequences, pSc119.2 (119), GAA microsatellite (GAA), and Afa family repeat (AFA), on A- and B-genome chromosomes of the durum wheat Langdon. (B–E) Chromosome arms sorted from ditelosomic lines of durum wheat after FISH with probes for GAA microsatellite (yellow-green) and telomeric repeat (red).