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. Author manuscript; available in PMC: 2006 May 1.
Published in final edited form as: Biochemistry. 2006 Apr 18;45(15):5029–5038. doi: 10.1021/bi0600704

Figure 5. Comparative effects of sphingolipids on the two steps of LCAT reaction.

Figure 5

Proteoliposome substrates containing 16:0-[1-14C]-18:2 PC and unlabeled cholesterol (at PC: cholesterol molar ratio of 20:1), and the indicated mol% of sphingolipid, were prepared as described in the text. After incubation with purified LCAT for 30 min, the lipids were extracted by ethanol/hexane, and the radioactivity in free fatty acids and CE was determined as described in Methods. All activities are expressed as percent of the activity obtained in the absence of added sphingolipid. Values shown are mean ± SEM of at least 3 experiments. Where indicated (** p< 0.005), the effect on phospholipase A activity was significantly different from the effect on FC esterification activity.