Skip to main content
. Author manuscript; available in PMC: 2006 May 1.
Published in final edited form as: Biochemistry. 2006 Apr 18;45(15):5029–5038. doi: 10.1021/bi0600704

Figure 6. Effect of ceramide on the fatty acid specificity of LCAT reaction.

Figure 6

Proteoliposomes containing an equimolar mixture of 16:0-16:0 PC, 16:0–18:1 PC, 16:0–18:2 PC, and 16:0–20:4 PC, labeled cholesterol (at total PC: FC ratio of 20:1), and 0, 5, or 15 mol% ceramide (with respect to total PC), were prepared as described in Methods. The reaction mixture was scaled up to contain 125 nmol of PC and 6.25 nmol of labeled cholesterol per reaction. After lipid extraction, 10% of the lipid extract was taken for the determination of total enzyme activity by TLC, and the rest was used for the separation of CE species by reverse phase HPLC as described in Methods. The activity of the enzyme (pmol CE formed/30 min) in this experiment was 412 ± 32 for control, 553 ± 47 for 5 mol% ceramide, and 606 ± 56 for 15 mol% ceramide. Values shown are mean ± SEM of 3 experiments. Statistical significance of difference between control and ceramide-containing substrates was determined by paired t test.

* p<0.05, ** p< 0.005.