Figure 4.—

Southern analysis of conservation of BACs 076J21 and 09M21 and conservation and methylation of subclones of 076J21. Lane numbers for all a–d refer to the species in Table 1. (a) BAC 076J21 and (b) 09M21 probed against the Glycine species blot. (c) Subclones of 076J21 probed against the Glycine species blots (only lanes showing hybridization are shown). (d) Methylation of 076J21 subclones containing Calypso 5-1 (1E_15) and STR102 (2P_01) were tested by probing against genomic DNA digested with either MspI or HpaII.