TABLE 2 .
Haploid yeast strains
| Strain | Relevant genotype | Construction details and/or reference |
|---|---|---|
| AS4-derived haploids | ||
| AS4 | Wild type | MATα trp1 arg4 tyr7 ade6 ura3 (Stapleton and Petes 1991) |
| TP1011 | rad1::ura3 | Kirkpatrick and Petes (1997) |
| DNY95 | Δpms1 | Kearney et al. (2001) |
| AS13-derived haploids | ||
| AS13 | Wild type | MATa leu2 ura3 ade6 (Stapleton and Petes 1991) |
| MW1 | his4-Sal | Nag et al. (1989) |
| DTK256 | rad1::ura3 | TST in MW1 with BamHI-digested pDG18 (Kirkpatrick and Petes 1997) |
| DTK509 | his4-F20R | TST in AS13 with SnaBI-digested pDTK139 |
| DTK609 | his4-F16 | TST in AS13 with SnaBI-digested pLEJ001 |
| DTK660 | his4-F15 | TST in AS13 with SnaBI-digested pLEJ003 |
| DTK662 | his4-F20 | TST in AS13 with SnaBI-digested pSLJ001 |
| DTK684 | his4-F17 | TST in AS13 with SnaBI-digested pSLJ003 |
| DTK695 | his4-F10 | TST in AS13 with SnaBI-digested pSLJ009 |
| DTK697 | his4-F14 | TST in AS13 with SnaBI-digested pSLJ010 |
| DTK739 | his4-F19 | TST in AS13 with SnaBI-digested pLEJ008 |
| DTK754 | his4-F18 | TST in AS13 with SnaBI-digested pLEJ007 |
| DTK859 | his4-F17R | TST in AS13 with SnaBI-digested pPAJ173 |
| DTK623 | his4-F16 rad1::ura3 | TST in DTK609 with BamHI-digested pDG18 |
| DTK677 | his4-F15 rad1::ura3 | TST in DTK660 with BamHI-digested pDG18 |
| DTK691 | his4-F20 rad1::ura3 | TST in DTK662 with BamHI-digested pDG18 |
| DTK716 | his4-F10 rad1::ura3 | TST in DTK695 with BamHI-digested pDG18 |
| DTK717 | his4-F14 rad1::ura3 | TST in DTK697 with BamHI-digested pDG18 |
| DTK727 | his4-F17 rad1::ura3 | TST in DTK684 with BamHI-digested pDG18 |
| DTK745 | his4-F19 rad1::ura3 | TST in DTK739 with BamHI-digested pDG18 |
| DTK770 | his4-F18 rad1::ura3 | TST in DTK754 with BamHI-digested pDG18 |
| DTK524 | his4-F20R Δpms1 | TST in DTK509 with BstXI-digested pJH523 (Kearney et al. 2001) |
| DTK678 | his4-F15 pms1::kanMX4 | OST in DTK660 with kanMX4; using primers 1305733 and 1305734 |
| DTK679 | his4-F20 pms1::kanMX4 | OST in DTK662 with kanMX4; using primers 1305733 and 1305734 |
| DTK713 | his4-F10 pms1::kanMX4 | OST in DTK695 with kanMX4; using primers 1305733 and 1305734 |
| DTK714 | his4-F14 pms1::kanMX4 | OST in DTK697 with kanMX4; using primers 1305733 and 1305734 |
| DTK715 | his4-F17 pms1::kanMX4 | OST in DTK684 with kanMX4; using primers 1305733 and 1305734 |
| DTK728 | his4-F16 pms1::kanMX4 | OST in DTK609 with kanMX4; using primers 1305733 and 1305734 |
| DTK731 | his4-F4 pms1::kanMX4 | OST in MW1 with kanMX4; using primers 1305733 and 1305734 |
| DTK744 | his4-F19 pms1::kanMX4 | OST in DTK739 with kanMX4; using primers 1305733 and 1305734 |
| DTK766 | his4-F18 pms1::kanMX4 | OST in DTK754 with kanMX4; using primers 1305733 and 1305734 |
| DTK881 | his4-F17R pms1::kanMX4 | OST in DTK859 with kanMX4; using primers 1305733 and 1305734 |
TST, two-step transplacement; OST, one-step transplacement.