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. 2005 Jul;170(3):1033–1043. doi: 10.1534/genetics.104.033670

TABLE 3 .

Effects of varying loop size and DNA repair activity on the meiotic segregation patterns of heterozygous markers at theHIS4 locus

No. of tetrads with various meiotic segregation patternsa
Strain HIS4 allele Loop
size		 Repair
mutation		 4:4 6:2 2:6 5:3 3:5 Ab
4:4		 7:1 1:7 8:0 0:8 Other
PMS		 Total
tetrads		 % Ab
seg		 %
unrepaired
MW103b  his4-Sal  4  WT 202 31 50  0  0 0 0 0 2 6 0 291 31  0
DTK257b  his4-Sal  4  rad1 249 66 70  0  0 0 0 0 4 3 0 392 36  0
DTK748  his4-Sal  4  pms1 142 19 15 23 16 2 2 0 1 0 1 221 36 54*
DTK696  his4-F10 10  WT 294 29 49  2  2 0 0 0 0 7 0 383 23  5
DTK721  his4-F10 10  rad1 185 35 82  1  6 0 0 3 0 2 0 314 41  7
DTK718  his4-F10 10  pms1 133 29  8 12 19 2 0 0 2 0 2 207 36 47*
DTK698  his4-F14 14  WT 178 22 30  2  2 0 0 0 1 1 1 237 25  8
DTK722  his4-F14 14  rad1 167 23 35  3 10 0 0 2 2 1 1 244 32 19
DTK719  his4-F14 14  pms1 137 28 10 17 25 1 0 2 1 0 0 221 38 52*
DTK661  his4-F15 15  WT 412 99 71  7  6 0 0 0 7 4 0 606 32  7
DTK680  his4-F15 15  rad1 182 43 33  5  4 0 0 0 0 1 0 268 32 10
DTK681  his4-F15 15  pms1 176 25 15 11 10 0 1 1 2 0 0 241 27 34*
DTK613  his4-F16 16  WT 168 22 26  1  1 0 0 0 0 0 0 218 23  4
DTK664  his4-F16 16  rad1 264 56 53 10 12 1 3 3 4 1 0 407 35 18*
DTK740  his4-F16 16  pms1 152 16  7 14 20 1 0 0 0 0 2 212 28 62*
DTK694  his4-F17 17  WT 264 43 30  8  7 1 0 2 0 1 0 356 26 18
DTK737  his4-F17 17  rad1 200 33 24 29 16 0 1 0 4 0 1 308 35 43*
DTK720  his4-F17 17  pms1 132 11 10 12 27 0 0 0 1 0 1 194 32 65*
DTK860  his4-F17R 17R  WT 165 32 21  1  0 0 0 0 3 0 0 222 26  2
DTK882  his4-F17R 17R  pms1 105 27 10  6  3 0 1 0 0 0 0 152 31 20*
DTK760  his4-F18 18  WT 140 35  9 16  3 0 1 1 4 1 0 210 33 29
DTK771  his4-F18 18  rad1 146 34 19 24  8 1 6 0 4 0 0 242 40 38
DTK768  his4-F18 18  pms1 153 30  7 17  6 0 1 1 0 0 0 215 29 39
DTK743  his4-F19 19  WT 224 31 25 12  4 0 0 0 1 1 0 298 25 22
DTK747  his4-F19 19  rad1 155 29 22 12  2 1 1 0 1 0 0 223 30 23
DTK746  his4-F19 19  pms1  83  8  6  2  5 1 0 0 0 0 1 106 22 39
DTK670  his4-F20 20  WT 311 38 22 17 37 1 1 1 0 1 1 430 28 48
DTK711  his4-F20 20  rad1 124 37 17 14 36 4 2 0 3 0 4 241 49 50
DTK705  his4-F20 20  pms1 145 15 14 11 19 1 0 2 0 1 0 208 30 51
DTK510  his4-F20R 20R  WT 495 57 40 16 18 2 0 2 3 1 1 635 22 27
DTK883  his4-F20R 20R  pms1 171 17 22  4  7 0 2 0 0 0 1 224 24 25
DNY27b  his4-lopd 26  WT 252 54 38 11  1 0 0 1 1 1 0 359 30 12
TP1013b  his4-lopd 26  rad1 294 86 28 37 24 3 4 2 3 0 0 481 39 36*
DTK309c  his4-lopd 26  pms1 238 40 45  6  0 1 0 0 1 2 0 333 29  7

% Ab seg, the percentage of total tetrads with an aberrant segregation pattern (non-4:4); % unrepaired, the number of unrepaired events (PMS tetrads) divided by the total number of aberrant segregation tetrads (PMS + GC), and expressed as a percentage. WT, wild type.

*

Significant (P < 0.05 or better) difference from wild type in the number of PMS tetrads vs. the number of GC tetrads.

a

For all segregation patterns, the first number represents the wild-type allele and the second, the mutant allele. The segregation patterns include: 4:4 (normal Mendelian segregation), 6:2 and 2:6 (gene conversion), 5:3 and 3:5 (tetrads with a single PMS event), Ab 4:4 (aberrant 4:4; one wild-type, one mutant, and two sectored colonies), 7:1 and 1:7 (tetrads yielding three spore colonies of one genotype and one sectored colony), and 8:0 and 0:8 (tetrads yielding four spores of a single genotype). The “Other PMS” class includes aberrant 6:2 and 2:6 tetrads as well as tetrads with three PMS events.

c

Data from Kearney et al. (2001).