TABLE 2 .
PCR and Southern blot analyses used to identify sharedrcb alleles in 13 strains ofC. cinereus with differentB specificities
| Hybridizationa/PCR amplification with strains of different B specificity |
||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Primer pair | Expected product (kb) |
B1 | B3 | B5 | B6 | B7 | B12 | B40 | B42 | B43 | B44 | B45 | B47 | B92 |
| rcb33 | 1.6 | +a | +a | − | − | − | +a | − | − | − | +a | − | − | −a |
| rcb35 | 1.8 | − | − | + | − | − | − | − | − | − | − | − | − | − |
| rcb36 | 1.6 | − | − | − | +a | − | − | +a | − | − | − | − | − | +a |
| rcb342 | 1.7 | − | − | − | − | +a | −a | − | +a | − | − | − | − | −a |
| rcb343 | 1.8 | − | − | − | − | − | − | − | − | + | − | − | − | − |
| rcb21 | 1.5 | + | − | − | − | − | − | − | − | − | − | − | + | − |
| rcb26 | 2.6 | − | − | − | +a | − | +a | − | − | − | − | − | − | +/−a |
| rcb242 | 1.5 | − | +a | − | − | − | − | − | +a | − | − | − | − | − |
| rcb243 | 2.0 | − | − | + | − | − | − | − | − | + | − | − | − | − |
| rcb244 | 1.5 | − | +/− | − | − | + | − | + | − | − | + | + | − | + |
| rcb13 | 2.1 | − | +a | − | − | +a | − | +a | − | +a | − | +a | − | − |
| rcb142 | 2.6 | +a | − | +a | +a | − | +a | − | +a | − | +a | − | +a | +a |
+, PCR amplification of a fragment of expected size confirmed as rcb by end sequencing; −, no PCR amplification; +/−, PCR amplification of a fragment of expected size but confirmed as not rcb by end sequencing.
a
Hybridization in Southern blot analyses.