Figure 4.—

Variable sites for a 2053-bp fragment of the tolkin gene from the St chromosomes (first 13 sequences) and the corresponding regions from both the functional (second 13 sequences) and inactivated (ψ) tok genes in In(3R)P chromosomes (see Figures 2 and 3). Base 1 of this fragment is the first base distal to the distal breakpoint. Coding sites are highlighted: polymorphic sites in the proregion of the protein are highlighted gray; those in the rest of the coding sequence are highlighted blue. Nonsynonymous changes are shown in boldface type. The underlined change is to a stop codon. The boldface numbers refer to insertion/deletion events: 1, a variable length poly(C) string; 2, a 12-bp deletion; 3, a 17-bp insertion; 4, an 11-bp insertion; 5, a 46-bp deletion; 6, a 142-bp deletion; 7, an 8-bp deletion. Red stars indicate the sites unique to the In(3R)P sequences. The reference sequence (“Genome”) is from the annotated D. melanogaster genome, release 3.2.2 (Adams et al. 2000; http://www.fruitfly.org).