Figure 3. Complement requirements for C3 deposition induced by CO antigen (1,000 ng/well).

Analysis of MBL-deficient serum (A) and C2-deficient, MBL-sufficient serum (B). The sera were C1qDP depleted and reconstituted with C1q (70 mg/l), D (1 mg/l), and P (25 mg/l) added alone or in combinations. Sera were used at a final concentration of 25%. Interpretations are shown at top. Each experiment was performed in duplicate and was repeated once with similar results.