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. 2006 May 1;116(5):1425–1434. doi: 10.1172/JCI25982

Figure 7. Influence of fractionated MBL/MASP complexes on C3 deposition induced by CO antigen in the serum of C2D:21.

Figure 7

Purified MBL/MASP complexes were separated according to charge and size on a Mono Q column. Elution patterns are shown by Western blot analysis of samples of the fractions as indicated above the blot (2 fractions were combined for application to each slot of the gel). The blot illustrates the differential elution of the MBL oligomers and the elution of the associated MASPs and MAp19. The different MBL oligomers are given as I–IV in order of increasing size (6). The analysis was performed with (open circles) and without (filled circles) reconstitution of the serum with purified C2 (6.5 mg/l). The experiment was repeated once with similar results.