After 2 weeks of external carotid arterial ligation, morphometric analysis was performed in perfusion-fixed RCAs and LCAs from WT (n = 6), Cav-1 KO (n = 11), and Cav-1 RC (n = 9) mice. (A) Morphometric analysis showed a reduction in the lumen diameter of LCAs compared with contralateral RCAs in WT and Cav-1 RC mice, with no changes in lumen diameter in Cav-1 KO mice, in response to a reduced flow remodeling stimulus. Wall thickness remained constant in remodeled LCAs from WT and Cav-1 RC mice, whereas wall thickness increased in LCAs of Cav-1 KO mice. (B and C) Representative images of H&E- (B) or trichrome-stained (C) cross sections of LCAs isolated from WT, Cav-1 KO, and Cav-1 RC mice showed obvious increased wall thickness in remodeled Cav-1 KO arteries. Magnification, ×400. (D) Increased BrdU incorporation into remodeled LCAs from Cav-1 KO mice. Values are mean ± SEM. *P < 0.05, 1-way ANOVA with Bonferroni post test.