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Specificities of cytokine determination in a multiplex system. A complete set of 15 microspheres were mixed with all biotinylated antibodies and one single standard (2,500 pg/ml). (A) MFI was measured using the Bio-Plex system for IL-10 at 2,500 pg/ml; (B and C) comparison of Bio-Plex and ELISA results. Shown are regression lines for IL-1β (correlation coefficient, 0.96) (B) and IL-6 (correlation coefficient, 0.85) (C).
