TABLE 1.
Reagents used for Bio-Plex immunoassaysa
| Cytokine | Recombinant protein
|
Capture Ab
|
Detection Ab
|
|||||
|---|---|---|---|---|---|---|---|---|
| Catalogue no. | Source | Catalogue no. | Clone | Source | Catalogue no. | Clone | Source | |
| IL-1α | SD901 | BS | AHC0912 | 624B 3F2 | BS | AHC0419 | 840C 20B8 | BS |
| IL-1β | M6058 | CLB | M9313 | CLB/IL1B-8 | CLB | M9313 | CLB/IL1B-4 | CLB |
| IL-2 | 554603 | BD | 555051 | 5344.111 | BD | 555040 | B33-2 | BD |
| IL-4 | 204-IL-005 | R&D | MAB604 | 3010.211 | R&D | BAF204 | Polyclonal | R&D |
| IL-5 | PHC0055 | BS | AHC0954 | JES1-39D10 | BS | AHC0859 | JES1-5A10 | BS |
| IL-6 | M6077 | CLB | M9316 | CLB/IL6-16 | CLB | M9316 | Polyclonal | CLB |
| IL-8 | 89/520 | NIBCS | M9318 | CLB/IL8-1 | CLB | M9318 | Polyclonal | CLB |
| IL-10 | M6086 | CLB | M9310 | B-N10 | CLB | M9310 | B-T10 | CLB |
| IL-12p70 | 219-IL-005 | R&D | MAB611 | 24945.11 | R&D | BAF219 | Polyclonal | R&D |
| IL-13 | 94/622 | NIBCS | M9313 | CLB/IL13-1 | CLB | M9313 | CLB/IL13-1 | CLB |
| IL-15 | 247-IL-005 | R&D | MAB647 | 34505.11 | R&D | BAM247 | 34593.11 | R&D |
| IL-17 | 317-IL-050 | R&D | MAB317 | 41809.111 | R&D | BAF317 | Polyclonal | R&D |
| IL-18 | B001-5 | MBL | D044-3 | 125-2H | MBL | D045-6 | 159-12B | MBL |
| TNF-α | M6145 | CLB | M9323 | CLB/TNF-7 | CLB | M9323 | CLB/TNF-5 | CLB |
| IFN-γ | 554616 | BD | 554548 | NIB42 | BD | 554550 | 4S.B3 | BD |
a
The reagents listed were obtained from the sources indicated by the following abbreviations: BS, Biosource; CLB, Sanquin; NIBSC, National Institute for Biological Standards and Controls (Potters Bar, United Kingdom); BD, BD Biosciences; R&D, R&D Systems; MBL, Medical & Biological Laboratories (Naka-ku Nagoya, Japan). All capture antibodies are mouse or rat monoclonal reagents. Detection antibodies for IL-4, IL-6, IL-8, IL-12, and IL-17 are either sheep or goat; others are monoclonal mouse or rat. Ab, antibodies.