TABLE 2.
Dynamic concentration ranges of ELISA and the Bio-Plex systema
| Cytokine | Dynamic concn range (pg/ml)
|
|
|---|---|---|
| ELISA | Bio-Plex | |
| IL-1α | 3.3-800 | 2.4-5,000 |
| IL-1β | 3.6-300 | 2.4-5,000 |
| IL-2 | 31.2-2,000 | 4.8-5,000 |
| IL-4 | 0.8-50 | 39-2,500 |
| IL-5 | 7.8-1,000 | 9.8-5,000 |
| IL-6 | 2.0-450 | 4.8-5,000 |
| IL-8 | 6.1-480 | 30-10,000 |
| IL-10 | 3.1-600 | 4.8-10,000 |
| IL-12p70 | 7.8-500 | 4.8-10,000 |
| IL-13 | 1.3-250 | 4.8-5,000 |
| IL-15 | ND | 9.8-1,000 |
| IL-17 | ND | 9.8-10,000 |
| IL-18 | 15.6-1,000 | 2.4-5,000 |
| TNF-α | 4.1-1,000 | 2.4-5,000 |
| IFN-γ | 5.1-500 | 4.8-5,000 |
a
Dynamic concentration ranges of the linear part of the standard curve of each cytokine were measured with ELISA or in a multiplex assay using the Bio-Plex system. ND, not done.