TABLE 3.

Recovery of spiked cytokines in a multiplex assay^a

Cytokine	Cytokine recovery (pg/ml) at expected concn indicatedb
	10 pg/ml	100 pg/ml	1,000 pg/ml
IL-1α	10.5 ± 1.3	97 ± 6	1,017 ± 51
IL-1β	10.1 ± 1.3	102 ± 12	1,020 ± 47
IL-2	9.6 ± 1.1	100 ± 8	1,003 ± 57
IL-4	17.0 ± 5.6	116 ± 19	998 ± 76
IL-5	12.1 ± 3.7	108 ± 7	991 ± 66
IL-6	10.4 ± 2.2	102 ± 19	1,003 ± 58
IL-8	25.2 ± 16.1	97 ± 10	1,048 ± 87
IL-10	9.3 ± 2.2	105 ± 5	1,014 ± 54
IL-12p70	11.9 ± 3.1	102 ± 11	978 ± 60
IL-13	11.7 ± 1.5	105 ± 6	1,018 ± 31
IL-15	9.2 ± 1.1	94 ± 11	1,007 ± 47
IL-17	26.7 ± 15.8	108 ± 18	1,045 ± 100
IL-18	8.7 ± 1.2	94 ± 3.4	1,007 ± 81
TNF-α	11.6 ± 2.3	103 ± 12	1,042 ± 70
IFN-γ	8.1 ± 3.2	91 ± 6	1,073 ± 120

^aKnown amounts of cytokines were spiked into RPMI culture medium. Samples were treated as unknown samples and determined in a multiplex assay. The mean ± standard deviation recoveries (picograms per milliliter) for the different amounts of each cytokine are shown.

^bSix experiments were done for each spiked cytokine.