TABLE 5.

Brucella genes identified by STM

Gene	Function	Species	Virulence		References
			In vitro	In vivo
ntrY	Two-component system (nitrogen metabolism)	B. suis	Attenuated	NDa	59
glnD	Uridylyl transferase (nitrogen metabolism)	B. suis	Attenuated	ND	59
pgi	Phosphoglucose isomerase (glucose metabolism)	B. suis	Attenuated	ND	59
manB	Phosphomannomutase (LPS synthesis)	B. suis	Attenuated	ND	59
gpt	Guanine phosphoribosyltransferase (nucleotide synthesis)	B. suis	Attenuated	ND	59
LTTR-like	LysR family transcriptional regulator	B. suis	Attenuated	ND	59
carAB	Carbamoyl phosphate synthase (arginine and pyrimidine metabolism)	B. suis	Attenuated	ND	59
rpsA	Ribosomal protein S1	B. suis	Attenuated	ND	59
pyc	Pyruvate carboxylase (glucose metabolism)	B. suis	Attenuated	ND	59
leuA	Isopropylmalate synthase (leucine synthesis)	B. suis	Attenuated	ND	59
cysK	O-Acetylserine (thiol)-lyase (amino acid metabolism)	B. melitensis	Attenuated	Attenuated	97
cysI	Sulfite reductase (amino acid metabolism)	B. melitensis	Attenuated	Attenuated	97
metH	Homocysteine-N5-methyltetrahydrofolate transmethylase (amino acid metabolism)	B. melitensis	Attenuated	Attenuated	97
mgtB	Mg2+ pump (transporter)	B. melitensis	Attenuated	Attenuated	97
rbsC	Ribose permease (transporter)	B. melitensis	Attenuated	Attenuated	97
ugpA	Glycerol-3-phosphate permease (transporter)	B. melitensis	Attenuated	Attenuated	97
ccrM	Adenine DNA methyltransferase (DNA metabolism)	B. melitensis	Attenuated	Attenuated	97
dnaJ	Heat shock protein	B. melitensis	Attenuated	Attenuated	97
mtgA	Peptidoglycan transglycosylase (membrane metabolism)	B. melitensis	Attenuated	Attenuated	97
vsrB	Two-component system	B. melitensis	Attenuated	Attenuated	97
fliF	Flagellar MS ring	B. melitensis	Attenuated	Attenuated	97
gcvB	Glycine dehydrogenase	B. abortus	ND	Attenuated	75
gluP	Glucose/galactose permease	B. abortus	ND	Attenuated	75
wbkA	LPS synthesis	B. abrotus	ND	Attenuated	75
gltD	Glutamate synthase	B. abortus	ND	Attenuated	75

^aND, not determined.