TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Description | Reference or source |
|---|---|---|
| Strains | ||
| E. coli DH5α λ-pir | φ80dlacZΔM15 Δ(lacZYA-argF)U169 recA1 endA1 gyrA96 thi-I hsdR17 supE44 relA1 deoR λ-pir | 19 |
| E. coli S17-1 λ-pir | recA pro hsdR RP4-2-Tc::Mu-Km::Tn7 λ-pir | 11, 32 |
| P. fluorescens Pf0-1 | Wild type, Ampr | 7 |
| P. fluorescens Pf0-2x | Pf0-1 adnA::Sm/Sp derivative | This study |
| Plasmids | ||
| pRK2013 | Helper plasmid, Tra+ Kmr | 13 |
| pHRP315 | Plasmid containing 2-kb DraI Ω cassette, Smr Spr | 28 |
| pJB866 | Broad-host-range vector, Tcr | 4 |
| pPC100 | 1.6-kb adnA gene cloned into AflIII-EcoRI site of pJB866, Tcr | 6 |
| pPC101 | Deletion of 923-bp AflIII fragment from adnA on pPC100, Tcr | 6 |
| pPF1B | 6.5-kb EcoRI-BamHI fragment cloned into pBluescript, Ampr | 6 |
| pJS6DRA | pPF1B derivative with Ω cassette cloned into the PmlI site of adnA, Ampr Spr Smr | This study |
| pSR47s | Broad-host-range suicide vector containing sacB, Kmr | 22 |
| pSUP101Tn5-B22 | pSUP101 derivative with Tn5 element containing promoterless lacZ, Tcr Gmr | 32 |
| pSUSM | pSR47s derivative containing adnA::Sm/Sp, Kmr Spr Smr | This study |
| pSRadnA | 3-kb SacI fragment containing adnA cloned in pSR47s | This study |