TABLE 3.
Phenotypic characterization of aba mutants in P. fluorescens
| Strain or aba mutant | adnA | Swimming | Biofilm | Sand column adhesion assaya | Chemotaxisb | Flagellac | Fold increase effect of AdnA on gene expressiond |
|---|---|---|---|---|---|---|---|
| Pf0-1 | + | + | + | 99 ± 1 | + (33) | + | ND |
| Pf0-2x | − | − | − | 65 ± 20 | − | − | ND |
| Pf0-2x (adnA) | + | + | + | 92 ± 0.7 | + (28) | + | ND |
| aba18 | − | − | + | 80 ± 15 | − | − | |
| + | + | + | 97 ± 1 | + (10) | + | 6 | |
| aba51 | − | − | + | 64 ± 6 | − | − | |
| + | + | + | 94 ± 2 | + (12) | + | 8 | |
| aba120 | − | − | − | 47 ± 23 | − | − | |
| + | − | + | 63 ± 20 | − | − | 64 | |
| aba160 | − | − | + | 27 ± 8 | − | − | |
| + | − | + | 58 ± 2 | − | − | 56 | |
| aba175 | − | − | − | 62 ± 8 | − | − | |
| + | − | + | 76 ± 6 | − | − | 34 (11) | |
| aba177 | − | − | − | 26 ± 12 | − | − | |
| + | − | + | 80 ± 14 | − | − | 25 | |
| aba203 | − | − | + | 38 ± 24 | − | − | |
| + | + | + | 88 ± 8 | + (16) | + | 35 (7.8) |
Values are mean percentages of cells that attached ± standard errors.
For strains exhibiting chemotaxis, the distance moved (in millimeters) is shown in parentheses.
Confirmed by electron microscopy.
Fold increase due to AdnA was estimated by β-galactosidase assays. All assays included the control consisting of aba strains harboring pPC101, which carries a deletion of the N-terminus domain of AdnA. Values shown are those obtained with adnA expressed from plasmid pPC100, apart from those in parentheses, which were determined from strains in which adnA::Sm/Sp was replaced by wild-type adnA (see Materials and Methods for details). LacZ activity for all insertion mutants was 2 to 7 Miller units, except for the aba175 mutant, which was 17 to 18 Miller units. ND, not determined.